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Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5309 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 5309 article reviews
Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5309 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+lines+vero+c1008/VERO+C1008/pm42243164-34-0-8
Average 99 stars, based on 5309 article reviews
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2026-09
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REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA-Seq of mice or HFF-1 cells infected with CHIKV and/or treated with cAIMP or Scleroglucan Gene Expression Omnibus Accession Number: GSE197744 Experimental models: Gene Expression:Article Title: Innate immune pathway modulator screen identifies STING pathway activation as a strategy to inhibit multiple families of arbo and respiratory viruses Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 2030-cGAMP Invivogen Cat#tlrl-nacga23 3030-cGAMP Invivogen Cat#tlrl-nacga AdilipolineTM (CL413) Invivogen Cat#tlrl-c413 C12-iE-DAP Invivogen Cat#tlrl-c12dap cAIMP Invivogen Cat#tlrl-nacai c-di-AMP Invivogen Cat#tlrl-nacda c-di-GMP Invivogen Cat#tlrl-nacdg FLA-BS Invivogen Cat#tlrl-bsfla FSL-1 Invivogen Cat#tlrl-fsl Gardiquimod Invivogen Cat#tlrl-gdqs iE-DAP Invivogen Cat#tlrl-dap Imiquimod Invivogen Cat#tlrl-imqs Loxoribine Invivogen Cat#tlrl-lox LPS-RS Invivogen Cat#tlrl-rslps LTA-BS Invivogen Cat#tlrl-lta MDP Invivogen Cat#tlrl-mdp ODN 4084-F Invivogen Cat#tlrl-4084 Pam2CSK4 Invivogen Cat#tlrl-pm2s-1 Pam3CSK4 Invivogen Cat#tlrl-pms Poly(A:U) Invivogen Cat#tlrl-pau Poly(dA:dT) naked Invivogen Cat#tlrl-patn Poly(dG:dC) naked Invivogen Cat#tlrl-pgcn Poly(I:C) (HMW) Invivogen Cat#tlrl-pic Poly(I:C) (LMW) Invivogen Cat#tlrl-picw Scleroglucan Invivogen Cat#tlrl-scg TL8-506 Invivogen Cat#tlrl-tl8506 Recombinant Human IFN-b Peprotech Cat#300-02BC Remdesivir (GS-5734) Selleckchem Cat#S8932 6-Azauridine MilliporeSigma Cat#A1882 Dimethyl sulfoxide MilliporeSigma Cat#D2650 RPMI 1640 Thermo Fisher Scientific Cat#11875093 B27 supplement with insulin Thermo Fisher Scientific Cat#17504044 CHIR-99021 (CT99021) Selleckchem Cat#S1263 IWR-1 MilliporeSigma Cat#I0161 Methanol (Histological) Thermo Fisher Scientific Cat#A433P4 16% Paraformaldehyde (formaldehyde) aqueous solution Electron Microscopy Sciences Cat#15710 Dulbecco’s Phosphate-Buffered Salt Solution 1X Corning Cat#21030CV DAPI (40,6-Diamidino-2-Phenylindole, Dihydrochloride) Thermo Fisher Scientific Cat#D1306 CorningTM Cell Culture Phosphate Buffered Saline (1X) Thermo Fisher Scientific Cat#MT21040CV Bovine Serum Albumin MilliporeSigma Cat#A9418 Normal Donkey Serum Jackson ImmunoResearch Cat#017-000-121 Normal Goat Serum Cell Signaling Technology Cat#5425S Triton X-100 MilliporeSigma Cat#T9284 Critical commercial assays CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#G7570 NAD/NADH-GloTM and NADP/NADPH-GloTM Assays Promega Cat#G9071 (Continued on next page) e2 Cell Reports Medicine 4, 101024, May 16, 2023 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA-Seq of mice or HFF-1 cells infected with CHIKV and/or treated with cAIMP or Scleroglucan Gene Expression Omnibus Accession Number: GSE197744 Experimental models: Derivative Assay:Article Title: Innate immune pathway modulator screen identifies STING pathway activation as a strategy to inhibit multiple families of arbo and respiratory viruses Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 2030-cGAMP Invivogen Cat#tlrl-nacga23 3030-cGAMP Invivogen Cat#tlrl-nacga AdilipolineTM (CL413) Invivogen Cat#tlrl-c413 C12-iE-DAP Invivogen Cat#tlrl-c12dap cAIMP Invivogen Cat#tlrl-nacai c-di-AMP Invivogen Cat#tlrl-nacda c-di-GMP Invivogen Cat#tlrl-nacdg FLA-BS Invivogen Cat#tlrl-bsfla FSL-1 Invivogen Cat#tlrl-fsl Gardiquimod Invivogen Cat#tlrl-gdqs iE-DAP Invivogen Cat#tlrl-dap Imiquimod Invivogen Cat#tlrl-imqs Loxoribine Invivogen Cat#tlrl-lox LPS-RS Invivogen Cat#tlrl-rslps LTA-BS Invivogen Cat#tlrl-lta MDP Invivogen Cat#tlrl-mdp ODN 4084-F Invivogen Cat#tlrl-4084 Pam2CSK4 Invivogen Cat#tlrl-pm2s-1 Pam3CSK4 Invivogen Cat#tlrl-pms Poly(A:U) Invivogen Cat#tlrl-pau Poly(dA:dT) naked Invivogen Cat#tlrl-patn Poly(dG:dC) naked Invivogen Cat#tlrl-pgcn Poly(I:C) (HMW) Invivogen Cat#tlrl-pic Poly(I:C) (LMW) Invivogen Cat#tlrl-picw Scleroglucan Invivogen Cat#tlrl-scg TL8-506 Invivogen Cat#tlrl-tl8506 Recombinant Human IFN-b Peprotech Cat#300-02BC Remdesivir (GS-5734) Selleckchem Cat#S8932 6-Azauridine MilliporeSigma Cat#A1882 Dimethyl sulfoxide MilliporeSigma Cat#D2650 RPMI 1640 Thermo Fisher Scientific Cat#11875093 B27 supplement with insulin Thermo Fisher Scientific Cat#17504044 CHIR-99021 (CT99021) Selleckchem Cat#S1263 IWR-1 MilliporeSigma Cat#I0161 Methanol (Histological) Thermo Fisher Scientific Cat#A433P4 16% Paraformaldehyde (formaldehyde) aqueous solution Electron Microscopy Sciences Cat#15710 Dulbecco’s Phosphate-Buffered Salt Solution 1X Corning Cat#21030CV DAPI (40,6-Diamidino-2-Phenylindole, Dihydrochloride) Thermo Fisher Scientific Cat#D1306 CorningTM Cell Culture Phosphate Buffered Saline (1X) Thermo Fisher Scientific Cat#MT21040CV Bovine Serum Albumin MilliporeSigma Cat#A9418 Normal Donkey Serum Jackson ImmunoResearch Cat#017-000-121 Normal Goat Serum Cell Signaling Technology Cat#5425S Triton X-100 MilliporeSigma Cat#T9284 Critical commercial assays CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#G7570 NAD/NADH-GloTM and NADP/NADPH-GloTM Assays Promega Cat#G9071 (Continued on next page) e2 Cell Reports Medicine 4, 101024, May 16, 2023 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA-Seq of mice or HFF-1 cells infected with CHIKV and/or treated with cAIMP or Scleroglucan Gene Expression Omnibus Accession Number: GSE197744 Experimental models: In Vivo:Article Title: Innate immune pathway modulator screen identifies STING pathway activation as a strategy to inhibit multiple families of arbo and respiratory viruses Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 2030-cGAMP Invivogen Cat#tlrl-nacga23 3030-cGAMP Invivogen Cat#tlrl-nacga AdilipolineTM (CL413) Invivogen Cat#tlrl-c413 C12-iE-DAP Invivogen Cat#tlrl-c12dap cAIMP Invivogen Cat#tlrl-nacai c-di-AMP Invivogen Cat#tlrl-nacda c-di-GMP Invivogen Cat#tlrl-nacdg FLA-BS Invivogen Cat#tlrl-bsfla FSL-1 Invivogen Cat#tlrl-fsl Gardiquimod Invivogen Cat#tlrl-gdqs iE-DAP Invivogen Cat#tlrl-dap Imiquimod Invivogen Cat#tlrl-imqs Loxoribine Invivogen Cat#tlrl-lox LPS-RS Invivogen Cat#tlrl-rslps LTA-BS Invivogen Cat#tlrl-lta MDP Invivogen Cat#tlrl-mdp ODN 4084-F Invivogen Cat#tlrl-4084 Pam2CSK4 Invivogen Cat#tlrl-pm2s-1 Pam3CSK4 Invivogen Cat#tlrl-pms Poly(A:U) Invivogen Cat#tlrl-pau Poly(dA:dT) naked Invivogen Cat#tlrl-patn Poly(dG:dC) naked Invivogen Cat#tlrl-pgcn Poly(I:C) (HMW) Invivogen Cat#tlrl-pic Poly(I:C) (LMW) Invivogen Cat#tlrl-picw Scleroglucan Invivogen Cat#tlrl-scg TL8-506 Invivogen Cat#tlrl-tl8506 Recombinant Human IFN-b Peprotech Cat#300-02BC Remdesivir (GS-5734) Selleckchem Cat#S8932 6-Azauridine MilliporeSigma Cat#A1882 Dimethyl sulfoxide MilliporeSigma Cat#D2650 RPMI 1640 Thermo Fisher Scientific Cat#11875093 B27 supplement with insulin Thermo Fisher Scientific Cat#17504044 CHIR-99021 (CT99021) Selleckchem Cat#S1263 IWR-1 MilliporeSigma Cat#I0161 Methanol (Histological) Thermo Fisher Scientific Cat#A433P4 16% Paraformaldehyde (formaldehyde) aqueous solution Electron Microscopy Sciences Cat#15710 Dulbecco’s Phosphate-Buffered Salt Solution 1X Corning Cat#21030CV DAPI (40,6-Diamidino-2-Phenylindole, Dihydrochloride) Thermo Fisher Scientific Cat#D1306 CorningTM Cell Culture Phosphate Buffered Saline (1X) Thermo Fisher Scientific Cat#MT21040CV Bovine Serum Albumin MilliporeSigma Cat#A9418 Normal Donkey Serum Jackson ImmunoResearch Cat#017-000-121 Normal Goat Serum Cell Signaling Technology Cat#5425S Triton X-100 MilliporeSigma Cat#T9284 Critical commercial assays CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#G7570 NAD/NADH-GloTM and NADP/NADPH-GloTM Assays Promega Cat#G9071 (Continued on next page) e2 Cell Reports Medicine 4, 101024, May 16, 2023 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA-Seq of mice or HFF-1 cells infected with CHIKV and/or treated with cAIMP or Scleroglucan Gene Expression Omnibus Accession Number: GSE197744 Experimental models: other:Article Title: Early IFN-α signatures and persistent dysfunction are distinguishing features of NK cells in severe COVID-19. Article Snippet: 50.000U MoBiTec Cat# GE-NUC10700-01 20% Human-Albumin Behring, salzarm CSL Behring Cat# PZN-01468366 BD Cytofix/Cytoperm BD Cat# 51-2090KZ BD Perm/Wash BD Cat# 51-2091KZ Cell Fix BD Cat# 340181 Spherotech ultra Rainbow beads SpheroTech Cat# URCP01-30-10K Critical commercial assays LIVE/DEAD Fixable Yellow Dead Cell Stain Kit Thermo Fisher Cat# L34967 Zombie aqua Biolegend Cat# 423102 LEGENDplex Human Inflammation Panel 1 (Mix&Match) Biolegend Cat# 740809 Human Single-Cell Multiplexing Kit Becton Dickinson Cat# 633781 BD Rhapsody WTA Amplification Kit Becton Dickinson Cat# 633801 BD Rhapsody Cartridge Kit Becton Dickinson Cat# 633733 BD Rhapsody cDNA Kit Becton Dickinson Cat# 633773 High Sensitivity D5000 ScreenTape Agilent Cat# 5067-5592 Qubit dsDNA HS Assay Kit ThermoFisher Cat# Q32854 Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit v3.1 10x genomics Cat# 1000121 Chromium Next GEM Chip G Single Cell Kit 10x genomics Cat# 1000120 Single Index Kit T Set A 10x genomics Cat# 1000213 High Sensitivity DNA Kit Agilent Cat# 5067-4626 NovaSeq 6000 S1 Reagent Kit (100 cycle) Illumina Cat# 200012865 NovaSeq 6000 S2 Reagent Kit (100 cycle) Illumina Cat# 20012862 NovaSeq 6000 S2 Reagent Kit (200 cycles) Illumina Cat# 20040326 NovaSeq 6000 S2 Reagent Kit (200 cycles) Illumina Cat# 20040326 NextSeq 500/550 High Output Kit v2.5 (150 Cycles) Illumina Cat# 20024907 Pan Monocyte Isolation Kit, human Miltenyi Cat# 130-096-537 CE/IVD Phagoburst BD Biosciences Cat# 341058 CD/IVD PHAGOTEST BD Biosciences Cat# 341060 NK Cell Isolation Kit, human Miltenyi Biotec Cat# 130-092-657 Deposited data scRNA-seq raw data This paper EGAS00001004571 Processed scRNA-seq count data and code This paper https://beta.fastgenomics.org/p/ Kraemer_2021_COVID19_NK Tables S1, S2, S3, and S4 This paper https://data.mendeley.com/datasets/ hwxhw2sxys/1 Experimental models: Cell lines VERO C1008 [Vero E6] ATCC Cat# Article Title: Early IFN-α signatures and persistent dysfunction are distinguishing features of NK cells in severe COVID-19 Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER STAR (Dobin et al., 2013) v2.6.1b Cutadapt (Martin, 2011) v1.16 Dropseq-tools https://github.com/broadinstitute/ Drop-seq/ v2.0.0 R https://www.cran.r-project.org v3.6.2 Seurat (R package) (Butler et al., 2018; Stuart et al., 2019) v3.1.4, v3.1.2 (CRAN) Harmony (R package) (Korsunsky et al., 2019) (https://github. com/immunogenomics/harmony) v1.0 Destiny (R package) (Angerer et al., 2016) v 3.0.1 ClusterProfiler (R package) (Yu et al., 2012) v3.10.1 (CRAN) SingleR (R package) (Aran et al., 2019) v1.0.5 (Bioconductor) DirichletReg (R package) (Maier, 2021) v0.6.3.1 (CRAN) AUCell (R package) (Aibar et al., 2017) v1.6.1 (CRAN) Cytobank (Chen and Kotecha, 2014); https://www.cytobank.org https://doi.org/10.1002/0471142956. cy1017s53 SPADE (Cytobank) (Qiu et al., 2011) Cytobank is running a version of SPADE derived from v1.10.2 flowCore (R package) https://www.bioconductor.org/ packages/release/bioc/html/ flowCore.html v1.48.1 (Bioconductor), 10.18129/ B9.bioc.flowCore CytoML (R package) https://github.com/RGLab/CytoML v1.8.1 (Bioconductor), 10.18129/ B9.bioc.CytoML CytofBatchAdjust (R package) https://github.com/CUHIMSR/ CytofBatchAdjust https://doi.org/10.3389/fimmu. Article Title: Transgenic mouse models support a protective role of type I IFN response in SARS-CoV-2 infection-related lung immunopathology and neuroinvasion. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Phosstop Roche Cat #4906845001 Picrosirius Red Stain kit Abcam Cat #ab150681 2X Laemmli Sample Buffer Bio-Rad Cat #1610737 Trypsin Gold Promega Cat #V5280 Trizol Invitrogen Cat #15596018 ProLong Gold Antifade Mountant Invitrogen Cat #P36930 Mouse M-CSF Recombinant Invitrogen Cat #PMC2044 DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) Invitrogen Cat #D1306 Phorbol 12-myristate 13-acetate, PMA Sigma Aldrich Cat #P8139 CP-456773 sodium salt, (MCC950) Sigma Aldrich Cat #PZ0280 Histopaque-1077 Sigma Aldrich Cat #10771 Evans Blue dye Sigma Aldrich Cat #E2129 Solvent Blue 38 Sigma Aldrich Cat #S3382 Trypan Blue solution Sigma Aldrich Cat #T8154 PMSF Sigma Aldrich Cat #P7626 Amido Black Staining Solution Sigma Aldrich Cat #A8181 VectaMount Permanent Mounting Medium Vector Laboratories Cat #H-5000 NP-40 Lysis buffer Thermofisher Scientific Cat #FNN0021 Critical commercial assays RNeasy Mini Kit Qiagen Cat #74104 Qubit dsDNA HS and BR Assay Kits Invitrogen Cat #Q32851 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat #4368813 Power SYBR Green PCR Master Mix Applied Biosystems Cat #A25742 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England BioLabs Cat #E7760L NEBNext Poly(A) mRNA Magnetic Isolation Module New England BioLabs Cat #E7490L BCA Protein Assay Kit Thermofisher Scientific Cat #23227 iTRAQ Reagents Multiplex Kit Sciex Cat #4352135 DAB Substrate Kit, Peroxidase (HRP), with Nickel Vector Laboratories Cat #SK-4100 Vector TrueVIEW Autofluorescence Quenching Kit Vector Laboratories Cat #SK-8400 Agilent 2200 High Sensitivity RNA Tape station Kit Agilent Technologies Cat #5067-5576, #5067-5577 and #5067-5578, Deposited data Raw FASTQ files for RNA Sequencing data This paper ArrayExpress database at EMBL-EBI with accession no.: E-MTAB-12681 Raw Proteomics files This paper Project accession: PXD044521 Project DOI: 10.6019/PXD044521 Western blot raw data This paper https://data.mendeley.com/https://doi.org/ 10.17632/59nr9kd5xy.1 Experimental models: Article Title: Early IFN-α signatures and persistent dysfunction are distinguishing features of NK cells in severe COVID-19 Article Snippet: 50.000U MoBiTec Cat# GE-NUC10700-01 20% Human-Albumin Behring, salzarm CSL Behring Cat# PZN-01468366 BD Cytofix/Cytoperm BD Cat# 51-2090KZ BD Perm/Wash BD Cat# 51-2091KZ Cell Fix BD Cat# 340181 Spherotech ultra Rainbow beads SpheroTech Cat# URCP01-30-10K Critical commercial assays LIVE/DEAD Fixable Yellow Dead Cell Stain Kit Thermo Fisher Cat# L34967 Zombie aqua Biolegend Cat# 423102 LEGENDplex Human Inflammation Panel 1 (Mix&Match) Biolegend Cat# 740809 Human Single-Cell Multiplexing Kit Becton Dickinson Cat# 633781 BD Rhapsody WTA Amplification Kit Becton Dickinson Cat# 633801 BD Rhapsody Cartridge Kit Becton Dickinson Cat# 633733 BD Rhapsody cDNA Kit Becton Dickinson Cat# 633773 High Sensitivity D5000 ScreenTape Agilent Cat# 5067-5592 Qubit dsDNA HS Assay Kit ThermoFisher Cat# Q32854 Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit v3.1 10x genomics Cat# 1000121 Chromium Next GEM Chip G Single Cell Kit 10x genomics Cat# 1000120 Single Index Kit T Set A 10x genomics Cat# 1000213 High Sensitivity DNA Kit Agilent Cat# 5067-4626 NovaSeq 6000 S1 Reagent Kit (100 cycle) Illumina Cat# 200012865 NovaSeq 6000 S2 Reagent Kit (100 cycle) Illumina Cat# 20012862 NovaSeq 6000 S2 Reagent Kit (200 cycles) Illumina Cat# 20040326 NovaSeq 6000 S2 Reagent Kit (200 cycles) Illumina Cat# 20040326 NextSeq 500/550 High Output Kit v2.5 (150 Cycles) Illumina Cat# 20024907 Pan Monocyte Isolation Kit, human Miltenyi Cat# 130-096-537 CE/IVD Phagoburst BD Biosciences Cat# 341058 CD/IVD PHAGOTEST BD Biosciences Cat# 341060 NK Cell Isolation Kit, human Miltenyi Biotec Cat# 130-092-657 Deposited data scRNA-seq raw data This paper EGAS00001004571 Processed scRNA-seq count data and code This paper https://beta.fastgenomics.org/p/ Kraemer_2021_COVID19_NK Tables S1, S2, S3, and S4 This paper https://data.mendeley.com/datasets/ hwxhw2sxys/1 Experimental models: Cell lines VERO C1008 [Vero E6] ATCC Cat# Article Title: Early IFN-α signatures and persistent dysfunction are distinguishing features of NK cells in severe COVID-19. Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER STAR (Dobin et al., 2013) v2.6.1b Cutadapt (Martin, 2011) v1.16 Dropseq-tools https://github.com/broadinstitute/ Drop-seq/ v2.0.0 R https://www.cran.r-project.org v3.6.2 Seurat (R package) (Butler et al., 2018; Stuart et al., 2019) v3.1.4, v3.1.2 (CRAN) Harmony (R package) (Korsunsky et al., 2019) (https://github. com/immunogenomics/harmony) v1.0 Destiny (R package) (Angerer et al., 2016) v 3.0.1 ClusterProfiler (R package) (Yu et al., 2012) v3.10.1 (CRAN) SingleR (R package) (Aran et al., 2019) v1.0.5 (Bioconductor) DirichletReg (R package) (Maier, 2021) v0.6.3.1 (CRAN) AUCell (R package) (Aibar et al., 2017) v1.6.1 (CRAN) Cytobank (Chen and Kotecha, 2014); https://www.cytobank.org https://doi.org/10.1002/0471142956. cy1017s53 SPADE (Cytobank) (Qiu et al., 2011) Cytobank is running a version of SPADE derived from v1.10.2 flowCore (R package) https://www.bioconductor.org/ packages/release/bioc/html/ flowCore.html v1.48.1 (Bioconductor), 10.18129/ B9.bioc.flowCore CytoML (R package) https://github.com/RGLab/CytoML v1.8.1 (Bioconductor), 10.18129/ B9.bioc.CytoML CytofBatchAdjust (R package) https://github.com/CUHIMSR/ CytofBatchAdjust https://doi.org/10.3389/fimmu. Blocking Assay:Article Title: Memory B cells targeting SARS-CoV-2 spike protein and their dependence on CD4 + T cell help Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human CD3-BV510 (clone UCHT1) Biolegend Cat# 300448, RRID: AB_2563468 Mouse anti-human CD19-APC-Cy7 (clone HIB19) Biolegend Cat# 302218, RRID: AB_314248 Mouse anti-human CD21-PE-Cy7 (clone Bu32) Biolegend Cat# 354912, RRID: AB_2561577 Mouse anti-human CD27-BV605 (clone O323) Biolegend Cat# 302830, RRID: AB_2561450 Mouse anti-human IgM-BV785 (clone MHM-88) Biolegend Cat# 314544, RRID: AB_2800832 Human anti-human IgA-VioBright 515 (clone REA1014) Miltenyi Biotec Cat# 130-116-886, RRID: AB_2727744 Mouse anti-human IgG-BV421 (clone G18145) BD Cat# 562581, RRID: AB_2737665 Mouse anti-human CD3-APC-Cy7 (clone UCHT1) Biolegend Cat# 300426, RRID: AB_830755 Mouse anti-human CD4-BV786 (clone SK3) BD Cat# 563877, RRID: AB_2738462 Mouse anti-human CD8-BV510 (clone RPA-T8) Biolegend Cat# 301048, RRID: AB_2561942 Mouse anti-human CD45RO-BV605 (clone UCHL1) Biolegend Cat# 304238, RRID: AB_2562153 Mouse anti-human CD40L-PE-CF594 (clone TRAP1) BD Cat# 563589, RRID: AB_2738297 Mouse anti-human CD279-PE-Cy7 (clone EH12.2H7) Biolegend Cat# 329918, RRID: AB_2159324 Mouse anti-human CXCR5-FITC (clone J252D4) Biolegend Cat# 356912, RRID: AB_2561894 Mouse anti-human IFNg-PE (clone B27) Biolegend Cat# 506507, RRID: AB_315440 Mouse anti-human IL4-BV421 (clone MP425D2) Biolegend Cat# 500826, RRID: AB_2561679 Mouse anti-human IL13-BV421 (clone JES10-5A2) Biolegend Cat# 501916, RRID: AB_2616748 Mouse anti-human AF647 (clone 3A3-N2) Biolegend Cat# 513006, RRID: AB_1227661 Bacterial and virus strains SARS-CoV-2 (Wuhan strain) Isolated from patient N/A Biological samples Peripheral blood of Covid-19-recovered individuals Heinsberg county, NRW, Germany N/A Peripheral blood of Covid-19-severely ill individuals University Hospital Bonn, Community Hospital Heinsberg, Germany N/A Chemicals, peptides, and recombinant proteins APC Streptavidin Biolegend Cat# 405207 PE Streptavidin Biolegend Cat# 405204 Biotinylated SARS-CoV-2 (COVID-19) S1 protein, His, Avitag (MALS verified) BioCat Cat# S1N-C82E8-200ug-AC DPBS, no calcium, no magnesium ThermoFisher Scientific Cat# 14190094 (Continued on next page) e1 Cell Reports 35, 109320, June 29, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER RPMI 1640 Medium ThermoFisher Scientific Cat# 21875034 DMEM, high glucose ThermoFisher Scientific Cat# 41965039 MEM Eagle Pan Biotech Cat# P03-2710 Sodium azide Sigma-Aldrich Cat# S2002-25G, CAS: 26628-22-8 EDTA (0.5 M), pH 8.0, RNase-free ThermoFisher Scientific Cat# AM9260G Penicillin-Streptomycin (5,000 U/mL) ThermoFisher Scientific Cat# 15070063 L-Glutamine (200 mM) ThermoFisher Scientific Cat# 25030081 BD GolgiStop BD Cat# 554724 BD GolgiPlug BD Cat# 555029 Ionomycin calcium salt Sigma-Aldrich Cat# I3909-1ML PMA Sigma-Aldrich Cat# P1585-1MG, CAS: 16561-29-8 Dimethyl sulphoxide (DMSO) Carl Roth Cat# A994.2 Pancoll human, Density: 1.077 g/ml Pan Biotech Cat# P04-60500 Sera Plus, EU approved regions, special processed FBS Pan Biotech Cat# P30-3702 Carboxymethylcellulose sodium salt Sigma-Aldrich Cat# C5013-1KG OptiPRO SFM ThermoFisher Scientific Cat# 12309019 Crystal violet Carl Roth Cat# T123.3 Ethanol Carl Roth Cat# T171.4 Formaldehyde solution 37% Carl Roth Cat# CP10.2 HEPES solution Sigma-Aldrich Cat# H0887-20ML Rainbow Fluorescent Particles Biolegend Cat# 422905 PepTivator SARS-CoV-2 Prot_S Miltenyi Biotec Cat# 130-126-700 PepTivator SARS-CoV-2 Prot_M Miltenyi Biotec Cat# 130-126-702 PepTivator SARS-CoV-2 Prot_N Miltenyi Biotec Cat# 130-126-698 Critical commercial assays Zombie Aqua Fixable Viability Kit Biolegend Cat# 423102 FcR Blocking Reagent, human Miltenyi Biotec Cat# 130-059-901 CD19 MultiSort Kit, human Miltenyi Biotec Cat# 130-055-301 Anti-SARS-CoV-2-ELISA (IgG) Euroimmun EI 2606-9601G Anti-SARS-CoV-2-ELISA (IgA) Euroimmun EI 2606-9601A Fixation/Permeabilization Solution Kit BD Cat# 554714 Experimental models: Software:Article Title: Memory B cells targeting SARS-CoV-2 spike protein and their dependence on CD4 + T cell help Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human CD3-BV510 (clone UCHT1) Biolegend Cat# 300448, RRID: AB_2563468 Mouse anti-human CD19-APC-Cy7 (clone HIB19) Biolegend Cat# 302218, RRID: AB_314248 Mouse anti-human CD21-PE-Cy7 (clone Bu32) Biolegend Cat# 354912, RRID: AB_2561577 Mouse anti-human CD27-BV605 (clone O323) Biolegend Cat# 302830, RRID: AB_2561450 Mouse anti-human IgM-BV785 (clone MHM-88) Biolegend Cat# 314544, RRID: AB_2800832 Human anti-human IgA-VioBright 515 (clone REA1014) Miltenyi Biotec Cat# 130-116-886, RRID: AB_2727744 Mouse anti-human IgG-BV421 (clone G18145) BD Cat# 562581, RRID: AB_2737665 Mouse anti-human CD3-APC-Cy7 (clone UCHT1) Biolegend Cat# 300426, RRID: AB_830755 Mouse anti-human CD4-BV786 (clone SK3) BD Cat# 563877, RRID: AB_2738462 Mouse anti-human CD8-BV510 (clone RPA-T8) Biolegend Cat# 301048, RRID: AB_2561942 Mouse anti-human CD45RO-BV605 (clone UCHL1) Biolegend Cat# 304238, RRID: AB_2562153 Mouse anti-human CD40L-PE-CF594 (clone TRAP1) BD Cat# 563589, RRID: AB_2738297 Mouse anti-human CD279-PE-Cy7 (clone EH12.2H7) Biolegend Cat# 329918, RRID: AB_2159324 Mouse anti-human CXCR5-FITC (clone J252D4) Biolegend Cat# 356912, RRID: AB_2561894 Mouse anti-human IFNg-PE (clone B27) Biolegend Cat# 506507, RRID: AB_315440 Mouse anti-human IL4-BV421 (clone MP425D2) Biolegend Cat# 500826, RRID: AB_2561679 Mouse anti-human IL13-BV421 (clone JES10-5A2) Biolegend Cat# 501916, RRID: AB_2616748 Mouse anti-human AF647 (clone 3A3-N2) Biolegend Cat# 513006, RRID: AB_1227661 Bacterial and virus strains SARS-CoV-2 (Wuhan strain) Isolated from patient N/A Biological samples Peripheral blood of Covid-19-recovered individuals Heinsberg county, NRW, Germany N/A Peripheral blood of Covid-19-severely ill individuals University Hospital Bonn, Community Hospital Heinsberg, Germany N/A Chemicals, peptides, and recombinant proteins APC Streptavidin Biolegend Cat# 405207 PE Streptavidin Biolegend Cat# 405204 Biotinylated SARS-CoV-2 (COVID-19) S1 protein, His, Avitag (MALS verified) BioCat Cat# S1N-C82E8-200ug-AC DPBS, no calcium, no magnesium ThermoFisher Scientific Cat# 14190094 (Continued on next page) e1 Cell Reports 35, 109320, June 29, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER RPMI 1640 Medium ThermoFisher Scientific Cat# 21875034 DMEM, high glucose ThermoFisher Scientific Cat# 41965039 MEM Eagle Pan Biotech Cat# P03-2710 Sodium azide Sigma-Aldrich Cat# S2002-25G, CAS: 26628-22-8 EDTA (0.5 M), pH 8.0, RNase-free ThermoFisher Scientific Cat# AM9260G Penicillin-Streptomycin (5,000 U/mL) ThermoFisher Scientific Cat# 15070063 L-Glutamine (200 mM) ThermoFisher Scientific Cat# 25030081 BD GolgiStop BD Cat# 554724 BD GolgiPlug BD Cat# 555029 Ionomycin calcium salt Sigma-Aldrich Cat# I3909-1ML PMA Sigma-Aldrich Cat# P1585-1MG, CAS: 16561-29-8 Dimethyl sulphoxide (DMSO) Carl Roth Cat# A994.2 Pancoll human, Density: 1.077 g/ml Pan Biotech Cat# P04-60500 Sera Plus, EU approved regions, special processed FBS Pan Biotech Cat# P30-3702 Carboxymethylcellulose sodium salt Sigma-Aldrich Cat# C5013-1KG OptiPRO SFM ThermoFisher Scientific Cat# 12309019 Crystal violet Carl Roth Cat# T123.3 Ethanol Carl Roth Cat# T171.4 Formaldehyde solution 37% Carl Roth Cat# CP10.2 HEPES solution Sigma-Aldrich Cat# H0887-20ML Rainbow Fluorescent Particles Biolegend Cat# 422905 PepTivator SARS-CoV-2 Prot_S Miltenyi Biotec Cat# 130-126-700 PepTivator SARS-CoV-2 Prot_M Miltenyi Biotec Cat# 130-126-702 PepTivator SARS-CoV-2 Prot_N Miltenyi Biotec Cat# 130-126-698 Critical commercial assays Zombie Aqua Fixable Viability Kit Biolegend Cat# 423102 FcR Blocking Reagent, human Miltenyi Biotec Cat# 130-059-901 CD19 MultiSort Kit, human Miltenyi Biotec Cat# 130-055-301 Anti-SARS-CoV-2-ELISA (IgG) Euroimmun EI 2606-9601G Anti-SARS-CoV-2-ELISA (IgA) Euroimmun EI 2606-9601A Fixation/Permeabilization Solution Kit BD Cat# 554714 Experimental models: |